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#1. Varioskan Flash 多功能判讀儀在DLR 檢測方面的應用
DLR(Dual-Luciferase Reporter, Promega)雙冷光酵素報導基因檢測系. 統是目前應用最為廣泛的化學冷光分析技術。本文將展示Varioskan Flash(.
冷光素酶(英語:Luciferase)是自然界中能夠產生生物發光的酶的統稱,其中最有代表 ... 活性;這一技術被稱為報告基因檢測法或冷光素酶檢測法(Luciferase Assay)。
Renilla Luciferase作为内参,来消除细胞数量、转染效率等的差异。Dual Luciferase Reporter Gene Assay Kit首先以萤光素为底物来检测萤火虫萤光素酶报告基因的活性,之后 ...
#4. Dual Luciferase Reporter Assay Kit_诺唯赞—科技成就健康生活
Dual Luciferase Reporter Assay Kit. 双萤光检测、结果更可靠. 萤光信号强. 检测范围宽. 灵敏度高. 分别构建Firefly luciferase 表达载体及Renilla luciferase 表达 ...
#5. Dual-Glo® Luciferase Assay System - Promega Corporation
The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two ...
#6. Dual Luciferase Assay System for Rapid Assessment of Gene ...
The dual luciferase assay is performed by sequentially measuring the firefly and Renilla luciferase activities of the same sample, with the results expressed as ...
Dual -Luciferase® Reporter,主要可分別測得Firefly luciferase和Renilla luciferase酵素活性,其中,測量Renilla luciferase冷光訊號,主要是用來當作Internal Control, ...
#8. TECAN 阿暉仔-: 雙色冷光(DLR) reporter assay
而雙色冷光(Dual luciferase reporter assay )的出現則解決了這方面的困擾 雙色冷光所採用的兩種不同色光的冷光蛋白分別是: 1. Firefly luciferase 是 ...
#9. Luciferase Assay - an overview | ScienceDirect Topics
In Dual-Glo® Luciferase Assay protocol, firefly luciferase is measured and quenched, followed by Renilla luciferase, in the same well. Performing most dual- ...
#10. luc assay 原理雙熒光素酶系統實驗操作步驟及方法 - Ccipmx
Dual Luciferase Reporter Gene Assay Kit 雙熒光素酶報告… Luciferase Reporter Gene Assay Kit 螢火蟲熒光素酶報告基因檢測試劑盒11401ES60/76/80 100/500/1000T 349/969 ...
#11. Dual Luciferase (Firefly-Renilla) Assay System - BPS Bioscience
The Dual Luciferase (Firefly-Renilla) Assay System is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a ...
#12. Dual Luciferase Reporter (DLR) assay certification - BMG ...
The dual luciferase assay is a fast reaction with 2 injection steps, one for the Firefly substrate (Luciferase Assay Reagent II or LAR II) and one for the Stop ...
#13. Dual-Luciferase® Reporter Assay System - YouTube
This video will describe the use of the Dual - Luciferase Reporter Assay System. Want to learn more? For additional resources, please visit ...
#14. Luciferase Assay Kit - ABP Biosciences
The ABP Dual Luciferase Assay Kit is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter ...
#15. Pierce™ Renilla-Firefly Luciferase Dual Assay Kit
The Thermo Scientific Pierce Renilla-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Renilla and ...
#16. Firefly & Renilla Luciferase Single Tube Assay Kit - Biotium
The Firefly & Renilla Luciferase Single Tube Assay is a combined luciferase assay allowing sequential measurement of Firefly and Renilla luciferase activity in ...
#17. 熒光素酶報告基因_百度百科
雙熒光素酶報告基因檢測(Dual- Luciferase reporter assay,DR)已成為研究轉錄因子 ... 應用原理. 轉錄因子是一種具有特殊結構、行使調控基因表達功能的蛋白質分子,也 ...
#18. Dual Luciferase Reporter Assay Kit (BA0180) 彙整 - 卓昇有限 ...
Dual Luciferase Reporter Assay Kit (BA0180). 首頁 / 商品Product Name / Dual Luciferase Reporter Assay Kit (BA0180). 找不到符合您選擇的商品 ...
#19. Firefly & Renilla Dual Luciferase Single Tube Assay Kit - Canvax
Firefly & Renilla Dual Luciferase Single Tube Assay is an extremely sensitive, accurate and fast Kit for the sequential quantitative measurement of both ...
#20. LucPair™ Duo-Luciferase Assay Kit - GeneCopoeia
The Luc-Pair™ Duo-Luciferase Assay Kit 2.0 has been improved upon from the Luc-Pair™ Duo-Luciferase Assay Kit. With enhanced stability, it provides a more ...
#21. Dual Luciferase Reporter Assay Kit - Vazyme
The Dual Luciferase Reporter Assay Kit is used to detect gene regulation by transfecting cells with a reporter plasmid and measuring the.
#22. 荧光素酶分析系统综述 - 来邦网
同义词: Dual Luciferase Assay System, Dual Luciferase kit, dual luciferase ... Dual-Luciferase报告分析系统被用于细胞转染来研究外异蛋白调控性变动调节棘鱼甲骨 ...
#23. TOOLShinning Dual Luciferase Reporter Assay Kit|圖爾思生技
Dual Luciferase Reporter Assay Kit can detect the expression of Luciferase regulated by gene elements sensitively and efficiently.
#24. Dual Luciferase Assay Kit - Creative Biogene
Creative Biogene's dual luciferase assay kit is the perfect choice for high sensitivity detection of Firefly luciferase (FLuc) and Renilla luciferase (Rluc) ...
#25. Dual Luciferase Reporter Assay Kit
In the assay, Firefly luciferase is detected with Luciferin as a substrate and then Renilla luciferase is detected with coelenterazine as a substrate. The Dual ...
#26. 荧光素酶酶活恢复法检测体内蛋白质互作 - Bio-protocol
实验原理:来自萤火虫的荧光素酶(Firefly luciferase, LUC)可以分成N和C两段 ... 酶活检测试剂盒Dual-Luciferase® Reporter Assay System (Promega, ...
#27. Dual Luciferase Reporter Assay Kit - 泓佑生物科技有限公司 ...
Overview, Dual Luciferase Reporter Assay Kit is used to detect gene regulation by transfecting cells with a reporter plasmid and measuring the fluorescence ...
#28. Dual Luciferase Reporter Gene Assay Kit - Antibodies-online ...
Order Dual Luciferase Reporter Gene Assay Kit (ABIN1000340) directly from us. ... Fast and convenient: three step assay allows detection of dual luciferase ...
#29. SuperLight™ Dual Luciferase Reporter Gene Assay Kit
Fast and convenient: three step assay allows detection of dual luciferase levels within 20 minutes. The accuracy of reporter assays can be improved by utilizing ...
#30. Application of the dual-luciferase reporter assay to the ...
The dual-luciferase assay has been widely used in cell lines to determine rapidly but accurately the activity of a given promoter.
#31. Dual Luciferase Reporter Gene Assay Kit 双荧光素 ... - 细胞生物
二者在翻译后均无需修饰即可发挥作用。 翊圣生物生产的Dual Luciferase Reporter Gene Assay Kit 双荧光素 ...
#32. Dual Luciferase Assay - Science method - ResearchGate
Dual luciferase assay is an everyday well assessed technique in my lab. I use a bicistronic plasmid carrying two ORFs coding for Renilla luciferase and Firefly ...
#33. Highly sensitive dual luciferase detection with the SpectraMax ...
Learn how dual luciferase detection with SpectraMax DuoLuc Reporter Assay Kit enables highly sensitive quantitation of firefly & Renilla luciferases in ...
#34. Dual-Luciferase® Reporter Assay System
The assays for firefly luciferase activity and Renilla luciferase activity are performed sequentially using one reaction tube. The following protocol is.
#35. 双萤光素酶报告基因检测试剂盒 - 生工
双萤光素酶报告基因检测试剂盒(Dual Luciferase Reporter Gene Assay Kit),即在一个系统中同时表达和测量两个独立的报告基因。先以萤光素(Luciferin)为底物来检测 ...
#36. Dual-Luciferase or Dual-Glo Luciferase Assay System? Which ...
The Dual-Luciferase® Reporter Assay System (DLR ® Assay) and Dual-Glo ® Luciferase Assay System accomplish the same thing: The sequential ...
#37. Dual-Luciferase Reporter Assay System E1910 from Promega
The Dual-Luciferase Reporter (DLR) Assay System provides an efficient means of performing two reporter assays. In the DLR Assay, the activities of firefly ...
#38. Dual Luciferase Reporter Gene Assay Kit - (KA3784) - Products
Dual Luciferase Reporter Gene Assay Kit is used for sequential measuring the activity of two different luciferases, firefly (FFL) and Renilla (RL), in a single ...
#39. An Optimized Transient Dual Luciferase Assay for Quantifying ...
The optimized dual luciferase assay provides an efficient, repeatable and scalable method to validate and quantify microRNA action on ...
#40. (曲线)Luciferase assay -v8.11 (1)
(Renilla luciferase), 同时抑制Firefly luciferase 的催化反应,实现双荧光素酶报告基因检测。Dual Luciferase Reporter Assay Kit 可灵敏.
#41. Bioluminescent Firefly Luciferase Assays - Sigma-Aldrich
This kit takes advantage of Firefly luciferase's use of ATP to oxidize D-Luciferin and the resulting production of light in to assess the amount of ATP ...
#42. Illumination™ Firefly & Renilla Luciferase Enhanced Assay Kit
Firefly and Renilla Luciferase Assays are two of the leading reporter assays in the measurement of gene function and gene regulation both in vitro as well ...
#43. Abnova™ Dual Luciferase Reporter Gene Assay Kit ...
Abnova Dual Luciferase Reporter Gene Assay Kit (Luminescence); Used for sequential measuring the activity of two different luciferases, firefly (FFL) and ...
#44. Dual Luciferase Reporter Gene Assay Kit - Antikoerper-Online ...
Can be readily automated on HTS liquid handling systems. Fast and convenient: three step assay allows detection of dual luciferase levels within 20 minutes.
#45. Luciferase Reporter Assay Kit (ab287865) - Abcam
Assay Firefly Luciferase in cell samples with Luciferase Reporter Assay Kit ab287865. Abcam offers > 1000 assay kits cited in > 3500 publications.
#46. Renilla-Firefly Luciferase Dual Assay Kit - MedChemExpress
MCE Renilla-Firefly Luciferase Dual Assay Kit is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a ...
#47. Dual Luciferase Reporter Gene Assay Kit 双荧光素 ... - 吉玛基因
Dual Luciferase Reporter Gene Assay Kit. 双荧光素酶报告基因检测试剂盒. 一.产品介绍. 双荧光素酶报告基因检测试剂盒,先以荧光素为底物来检测萤火虫荧光素酶报告 ...
#48. Luciferase Assay System Technical Bulletin #TB281
Store the supernatant/cell lysate at –70°C or proceed to Section IV. E. Protocol for Plant and Bacterial Cell Lysates and Tissue Homogenates. 1. For plant ...
#49. Luciferase reporters—Ready-To-Glow secreted luciferase assay
631740: Ready-To-Glow Automation Kit ... The Ready-To-Glow Dual Secreted Reporter Assay contains the buffers and substrates for use with the vectors contained in ...
#50. Twinlite Firefly & Renilla Dual Luciferase Reporter Assay
Detect and quantitate both Firefly and Renilla luciferase sequentially from cultured cells using twinlite dual luciferase reporter gene assay system.
#51. Dual-Spectral Luciferase Reporter Gene Assays | Agilent
Gaussia-Firefly Luciferase Dual Assay Kit (Cat. #16182) and cell lysates from cells expressing Gaussia or Firefly lucifer- ase or control cell lysate devoid of ...
#52. A Firefly Luciferase Dual Color Bioluminescence Reporter ...
Perhaps the most popular format, typified by the Promega Dual-Luciferase® Reporter (DLR ® ) assay system, involves two unrelated luciferases that ...
#53. Protocol – a promotor assay (Dual-Luciferase® Reporter
Protocol – a promotor assay (Dual-Luciferase® Reporter. Assay System):. Preparation: • You will need phosphate-buffered saline, a luminometer, ...
#54. Luciferase Reporter Assay Kit II (Renilla) - PromoCell
Renilla Luciferase has been used as a reporter gene for studying gene regulation ... PromoKine also offers the Luciferase Reporter Assay Kit III (Firefly ...
#55. BioLux® Gaussia Luciferase Assay Kit - NEB
The BioLux® Gaussia Luciferase Assay Kit contains the reagents necessary for assaying Gaussia Luciferase (GLuc) activity. This kit is most commonly used ...
#56. Dual Luciferase Reporter Assay Kit - Absource Diagnostics
Dual Luciferase Reporter Assay Kit. You are here: Home · Uncategorized; Dual Luciferase Reporter Assay Kit. Awaiting product image ...
#57. Luciferase Assay Kit (Luminescent) (KA1640) - Novus ...
Luciferase Reporter Gene Assay Kit is a bioluminescent assay for promoter ... Dual luciferase assay system for rapid assessment of gene expression in ...
#58. Luciferase reporter assays to monitor interferon signaling ...
We present a protocol for analyzing the impact of SARS-CoV-2 proteins in interferon signaling using luciferase reporter assays. Here, the ...
#59. Dual Luciferase Gene Reporter Assays to Study miRNA ...
This chapter describes one of the most reliable quantitative assays to test the silencing of a possible target gene by a specific miRNA using a luciferase ...
#60. Dual Luciferase Reporter Assay - Molecular Cloning
Assay System (Promega). It uses luciferase enzymes from the firefly (Photinus pyralis) and sea ...
#61. In vivo processing assay based on dual luciferase reporter ...
We developed a protocol that applies a dual luciferase assay to measure enzymatic activity of DROSHA enzyme. DROSHA is a member of ribonuclease III family ...
#62. Luciferase Assay - CSH Protocols
In this protocol, cells transfected with a luciferase reporter plasmid are lysed using a detergent-containing buffer. Cell debris is removed by ...
#63. Firefly & Renilla Luciferase Single Tube Assay Kit - Biotrend
This kit is a new and improved replacement for the Firefly & Renilla Dual Luciferase Assay Kit (catalog no. 30005). Other commercial luciferase assays require ...
#64. デュアルルシフェラーゼアッセイ原理 - Gustavob
Dual Luciferase Reporter Assay Kit可靈敏高效地檢測受基因元件調控的Luciferase ... プロメガのDual-Luciferase® Reporter (DLRTM) Assay System(a,b,c) を利用する ...
#65. High-throughput Functional Screening using a Homemade ...
We also include an inexpensive alternative to commercially available dual-luciferase reagents for high-throughput assays. This protocol can be ...
#66. Luc-Pair™ Luciferase Assay Kit | 复能基因
高灵敏性 Luc-Pair™ Duo-Luciferase HS Assay Kit 是一款高灵敏性双荧光素酶检测试剂盒,可高效、快速检测萤火虫(Firefly luciferase,FLuc)和海肾(Renilla ...
#67. Dual Luciferase Assay Kit (Promega) - Bioz
Dual Luciferase Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations.
#68. 实验笔记丨双荧光素酶报告基因验证法 - 知乎专栏
双荧光素酶报告基因系统(Dual-Luciferase® Reporter Assay System)是一种以使用荧光素(Luciferin)作为底物来测定萤火虫荧光素酶(Firefly ...
#69. デュアルレポーターアッセイ原理 - Luenen
同時抑制Firefly luciferase的催化反應,實現雙螢光素酶報告基因檢測。. Dual Luciferase Reporter Assay Kit可靈敏高效地檢測受基因元件調控的Luciferase.
#70. Dual-Luciferase Reporter Assay的概述|专业知识背景|拓展阅读-小狗 ...
其原理主要为:1)构建一个将靶启动子的特定片段插入到荧光素酶表达序列前方的报告基因质粒,如pGL3-basic等。2)将要检测的转录因子表达质粒与报告基因质粒共转染293细胞 ...
#71. Cell-Based Luciferase Reporter Assay Platform - INDIGO ...
Each kit includes luciferase reporter cells, an assay plate, all required reagents (optimized culture media, positive-control agonist, and detection reagent), ...
#72. LightSwitch™ Luciferase Assay System - Active Motif
The LightSwitch™ Luciferase Assay System from Active Motif is a complete solution for gene regulation studies in mammalian cells, with over 18000 pre-cloned ...
#73. デュアルレポーターアッセイ原理 - Sfartpo
Dual Luciferase Reporter Assay Kit-诺唯赞——科技成就健康生活-DualLuciferaseReporterAssayKit双萤光检测、结果更可靠萤光信号强检测范围宽灵敏度高分别 ...
#74. Dual-Glo萤光素酶检测系统- 泽浩公司
专业的报告基因检测试剂来自Promega —— 萤光素酶检测系统. ☆ 萤光素酶检测系统 (Luciferase Assay System). ☆ Dual-Luciferase® 双萤光素酶报告基因检测系统 ...
#75. Dual-Luciferase® Reporter Assay System 双荧光素酶实验
Dual - Luciferase ® Reporter Assay System 双荧光素酶实验. 677播放 · 总弹幕数02021-11-16 01:29:53. 主人,未安装Flash插件,暂时无法观看视频,您可以…
#76. CTB-193M12.5 promotes hepatocellular carcinoma progression
... assay, qPCR, Western blot, and dual luciferase reporter assay. ... After quantification using the Enhanced BCA Protein Assay Kit ...
#77. PpHYH is responsible for light-induced anthocyanin ...
punched to measure the firefly and ranilla luciferase fluores- cence intensity using Dual-Luciferase Reporter Gene Assay Kit. (Promega).
#78. Regulatory RNAs in the Nervous System, 2nd Edition
Luciferase was activated using the Dual-Luciferase Reporter Assay kit (Promega) using the manufacturer's protocoland read on a Victor3 1420 multilabel ...
#79. Combinatorial Approaches to Enhance Anti-Tumor Immunity: ...
Aluciferase assay was then performed using Dual luciferase assay kit (Promega). Fold changes were calculated as (P.I. stimulated Firefly Luciferase/Renilla ...
#80. Journal of the National Cancer Institute: JNCI.
Firefly luciferase To identify common pathways shared by miRNAs 221 , 222 , and 206 , activity was measured with a Dual Luciferase Assay Kit ( Promega ) as ...
#81. Cell-wide Metabolic Alterations Associated with Malignancy
3.1.4 Measure luciferase activity Twenty-four to forty-eight hours after the ... assay lucifer- ase activity using a Dual-Luciferase Reporter Assay kit ...
#82. miR‐24‐3p obstructs the proliferation and migration of human ...
293T was collected 48 h after gene transfection, and luciferase ... of the dual-luciferase reporting assay system (Promega, Madison, WI, ...
#83. Genes, Volume 13, Issue 6 (June 2022) – 82 articles - MDPI
Using luciferase reporter gene assays, we found significant cell type- and ... which was validated by dual luciferase reporter assay system and qRT-PCR.
#84. Mark Morris - Senior Scientist - Base Pair Biotechnologies, Inc.
Performed SELEX based assays on RNA internal loop libraries to determine sequence specific binding patterns of aminoglycosides.
#85. Expanded Liquid Chromatography-Mass Spectrometry ...
RNA immunoprecipitation, biotinylated RNA pull-down, ChIP, and dual-luciferase assays were employed to validate the regulatory network of ...
dual luciferase assay原理 在 Dual-Luciferase® Reporter Assay System - YouTube 的推薦與評價
This video will describe the use of the Dual - Luciferase Reporter Assay System. Want to learn more? For additional resources, please visit ... ... <看更多>